| BLK

Tyrosine-protein kinase BLK, also known as B lymphocyte kinase, is a non-receptor tyrosine kinase that is encoded by the BLK gene. It is of the Src family of tyrosine kinases.The tyrosine-protein kinase BLK has been shown to interact with UBE3A.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.19	1.61	1.05	0.66	0.43	0.29	0.21	0.07
Corrected OD	2.12	1.54	0.98	0.59	0.36	0.22	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human BLK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	95
Cell Culture Supernates(n=8)	93-105	102

 

| Linearity

Samples were spiked with high concentrations of Human BLK and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	92
1 : 4	Range (%)	90-103	87-108
	Average (%)	94	98